Generator
Part:BBa_K105016:Design
Designed by: Michael Wild, Katja Karstens Group: iGEM08_ESBS-Strasbourg (2008-10-22)
Leu2 - Beta-isopropylmalate dehydrogenase of yeast
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1296
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1296
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1296
Illegal XhoI site found at 2 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1296
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1296
Illegal AgeI site found at 909 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design. So there is no base in between the BioBrick and the restriction sites. <br\n>
The deletation of the internal EcoRI site would be possible with a silent mutation, but hasn't been done yet. So this part can only be used as Backinsert for the moment.
Source
Amplification from the plasmid pRS315 (Sikorski & Hieter)