Generator

Part:BBa_K105016:Design

Designed by: Michael Wild, Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-22)

Leu2 - Beta-isopropylmalate dehydrogenase of yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1296
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1296
    Illegal XhoI site found at 2
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1296
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1296
    Illegal AgeI site found at 909
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design. So there is no base in between the BioBrick and the restriction sites. <br\n>

The deletation of the internal EcoRI site would be possible with a silent mutation, but hasn't been done yet. So this part can only be used as Backinsert for the moment.

Source

Amplification from the plasmid pRS315 (Sikorski & Hieter)

References